以分子選殖方式建構 V2 C端表達載體系統並建構nociceptin receptor V2 tail 及 d opioid receptor receptor V2 tail 表達質體

G蛋白偶聯接受體藥物研發二年計畫

鴉片受體包含三種主要之亞種，分別是m、d及k。早期鴉片受體藥物之開發多針對疼痛之處理，事實上近年來的研究顯示，鴉片受體在腦部以外有許多新的潛在生理功能。本報告針對鴉片受體藥物進行研發，以delta鴉片受體為主要標的。本實驗包括兩個部分：第一，選殖d opioid receptor；第二，決定適當位置置換d opioid  receptor之C端，選殖入pNRKpuro-V2之載體。

Opiate receptor contains three main subspecies, namely m 、 d 、k 。 Early opiate receptor drugs developed for treatment of pain, and in fact recent studies showed that opioid receptors in the brain has a lot of potential new physiological functions. This report aimed at opioid receptors drug research and development, Delta opioid receptors as main subject. This study consists of two parts: first, the cloning d opioid receptorand second, decide on an appropriate replacement d opioid receptor c -side, cloning in pNRKpuro-V2 Of the carrier.